The core not only produces genetically modified mice but also helps design the DNA constructs for pronuclear microinjection or gene targeting with the most up-to-date genetic technologies such as the CRISPR/Cas9 genome editing system.
The core also offers assisted fertilization services followed by embryo transfer to save mutant mouse lines, as well as cryopreservation of embryos and sperm for conservation of genetic resources.
These in-house services reduce the effort and cost to Cedars-Sinai investigators and staff scientists by providing the scientific and technical expertise for the production, importation/exportation, maintenance and preservation of the genetically modified mice. The core encourages interactive usage of these animals among Cedars-Sinai investigators. This resource provides users the chance to obtain high-impact experimental results and more funding opportunities.
Since its launch in March 2011, the core has:
- Rederived 269 mutant mouse lines
- Injected 51 transgenic constructs (of which 9 are CRISPR constructs)
- Cryopreserved mouse embryos from 87 lines and sperm from 171 lines
- Genotyped 42 lines
- Established three embryonic fibroblast cell lines from one double knockout line
- Cryorecovered 20 (sperm) and 47 (embryos) mutant mouse lines
As of June 2018.