Rigorously established single or multiplexed immunoassays are available for an extensive list of analyte targets; assay development services are available for new target analytes.
State-of-the-art enzyme-linked immunosorbent assay (ELISA) quantification for protein quantification is provided using one of two ELISA platforms:
- Ultra-sensitive digital ELISA, SIMOA HD-1 Analyzer
- High-performance electrochemiluminescence ELISA platform, MESO QuickPlex SQ 12.
The MESO QuickPlex SQ 12 provides ease of multiplexing, ability to mount new analytes, large dynamic range and industry-leading sensitivity making it an excellent option for many studies. Quanterix is a newer ELISA platform; their Simoa™ HD-1 Analyzer can achieve single molecule detection, representing a leap in sensitivity for most assays compared with traditional antibody-based analyses. As a result, Quanterix technology is the most sensitive ELISA-type assay platform currently available.
The Core assists in study design and choice of existing commercial single and multiplex ELISA assays and/or in assay development to a new target analyte. ELISAs can be used to quantify one or more proteins simultaneously in serum, plasma and other body fluids, as well as in cell and tissue lysates. The Core also carries out aliquoting, processing samples, running samples and analyzing and reporting data. All assays are run in duplicate. Quality controls embedded in the method ensure that all assays are repeated if samples exceed a coefficient variant percentage (%CV) greater than 20%.