Research Services

Custom iPSC Generation Packages

Includes generation of clonal iPSC lines for a patient sample provided by customer and Complete or Essential pluripotency characterization package. Please contact us for prices and IRB clearance if an iPSC line needs to be derived from fresh skin biopsy.

Source
Package
Characterization Suite
Price

Fibroblasts

3 clones

Complete

$12,000

2 clones

Complete

$10,000

1 clone

Complete

$8,000

3 clones

Essential

$6,250

2 clones

Essential

$5,250

1 clone

Essential

$4,250

Source
Package
Characterization Suite
Price

LCLs or
peripheral blood

3 clones

Complete

$12,500

2 clones

Complete

$10,500

1 clone

Complete

$8,500

3 clones

Essential

$6,750

2 clones

Essential

$5,750

1 clone

Essential

$4,750

Complete Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluirpotency Markers, G-Band Karyotype, RT-qPCR for Endogenous Pluripotency gene expression, hPSC Scorecard Assay, EBNA1 gDNA PCR, Cell Line Authentication (STR)

Essential Characterization Package includes: Mycoplamsa Testing, AP Staining, ICC for Pluripotent Markers, G-Band Karyotype, Cell Line Authentication (STR)

Source tissues can include: fibroblast (skin), fresh whole blood (PBMCs), MSCs, adipose cells, various stromal epithelial cell types, and lymphoblastoid cells lines (LCLs or EBV B-cells). The fresh blood must be collected in CPT tubes and centrifuged according to manufacturer's directions. All reprogramming is preformed using non-integrating techniques in defined media (serum and KSR-free) and feeder-free conditions (no MEFs). Cost for each patient line includes the expansion and banking of three thoroughly characterized clonal lines under feeder-free conditions. Each cell line will be accompanied by a rigorous Certificate of Analysis to ensure pluripotency, normal karyotype, embryoid body formation and a variety of services related to the characterization of the pluripotent state (see below).

Pluripotent Stem Cell (PSC) Quality Control and Characterization Services

The RMI iPSC Core offers a variety of assays to ensure the quality and pluripotency of PSC lines. PSC characterization is included in the custom iPSC generation service, or separately as needed. Please contact the iPSC Core for more information on any of our individual characterization services.

Service
Requirements
Price / iPSC Line (1 clone)

Mycoplasma Testing

Please provide the core with at least 1ml of 2-day-old conditioned media. The core regularly performs mycoplasma testing once a month. Contact the core for the next available mycoplasma testing date.

$17

Alkaline Phosphatase (AP) Staining

Please provide the core with 1 well of 50-60% confluent cells.

$40

Immunocytochemistry (ICC) staining
SSEA4 + OCT4  
TRA-1-60 + NANOG
TRA-1-81 + SOX2

Please proved the core with 1 well of good quality cells that are at least 50-60% confluent.

$80

G-Band Karyotype

Please provide the core with 2 80% confluent wells

$450

PluriTest Assay- Currently Unavailable

Please provide the core with 1-2 80% confluent wells.

$320

RT-qPCR for endogenous/exogenous pluripotency gene expression

Please provide the core with 1-2 wells of good quality cells that are at lease 80% confluent

$100

Embryoid Body (EB) formation, RT-PCR gene expression for 3 lineages

Please provide the core with a 70-80% confluent 6-well plate of cells.

$340

Please provide the core with a 70-80% confluent 6-well plate of cells. Price is per clone. Please provide the core with a 70-80% confluent 6-well plate of cells. We will generate EBs and also test parent iPSCs. This assay is done in pair of iPSCs + EBs generated from the iPSCs for 10-18 days. Price reflected is for the pair.

$600

EBNA1 gDNA PCR

Confirm lack of exogenous plasmid presence. Price is per clone.

$200

Biobanking and PSC Thawing

As part of the custom iPSC generation service, the core will expand and bank three fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.

Service
Service Description
Requirements
Price

Cell Line Cryopreservation

Guaranteed cryopreservation of high quality, mycoplasma negative cells (6 vials total-feeder free).

Please provide the core with 1 well of starter cells.

$150

PSC Thaw

The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and maintain cells for 1 week.

Please provide the core with 1 vial of cryopreserved cells.

$50

PSC Thaw and Expansion

The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and expand cells into a full 6-well plate over the course of 2 weeks.

Please provide the core with 1 vial of cryopreserved cells.

$150

PSC Research Grade Biobank

The core will generate a research grade bank of multiple cryopreserved vials of PSCs.

Karyotype will be performed prior to initiating banking and at the time of creating a research grade lot.

PBMC Isolation and Processing
Service
Requirements
Price/ Sample

Peripheral Blood Mononuclear Cell (PBMC) Isolation and Processing

Fresh whole blood collected in 8ml BD CTP Vacutainer tubes

$300

The iPSC Core will isolate peripheral blood mononuclear cells (PBMCs) from freshly collected whole blood. Cells will be cryopreserved at 5,000,000 cells per vial. The iPSC Core recommends collecting a minimum of two (2) 8ml CPT Vacutainer tubes. Please see the references below for more information on the collection of whole blood as well as the shipment of samples to Cedars-Sinai or contact us.

Browse Cell Line Catalog

Hundreds of already established lines spanning over 30 degenerative diseases.